Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Abcam PBS Tablets (1000 ml), 100TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Research Products International Corp PBS, 1X Solution, 1.0ml Pre-Filled Tubes, S, 1000 CS
PBS (Phosphate-Buffered Saline) is a balanced salt solution that helps maintain the pH and osmolarity of experimental systems, making it ideal for various biological and biochemical experiments.
1X Solution refers to a diluted concentration of a particular solution. In the case of PBS, a 1X solution means that it is prepared by diluting the concentrated stock solution to a specific ratio, usually 1 part stock solution to 9 parts water or another suitable solvent. 1.0ml Pre-Filled Tubes are small tubes that come pre-filled with a specified volume of liquid, in this case, 1.0 milliliters of PBS. These tubes are convenient and sterile, ensuring the integrity and purity of the solution. They are commonly used in laboratory settings to provide a controlled and standardized amount of PBS for various applications.
Abcam Tris-HCl (pH 8.8), 100ML
A 1.5 M solution of Tris HCl in water (pH 8.8).
The product is subject to the following: Abcam Restricted Use Statement
Research Products International Corp DIPSO 100 G
Substance Name: 3-(N,N-Bis(2-Hydroxyethyl)amino)-2-hydroxypropane sulfonic acid
CAS Number: 68399-80-4
Molecular Formula: C7H17NO6S
Molecular Weight: 243.3
Chemical Purity: >98%
Soluble in:?Water
Store at:?Room Temperature
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.0) - 250ml
Bicine Buffer (0 5 M pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Alkali Scientific CellPro™ PBS Buffer, 1X , pH 7.4, 20L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 7.5) - 250ml
Bicine Buffer (0 5 M pH 7 5) - 250ml (For Research Use and Further Manufacturing Only)
![Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75060-1000.0.JPG-250.jpg)
Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 1 Kilogram
- Substance Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
- CAS Number: 16052-06-5
- Molecular Formula: C9H20N2O4S
- Molecular Weight: 252.3
Teknova 1M Tris-HCl, pH 7.4. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova TE Buffer, pH 8.0. (10mM Tris, 1mM EDTA). 250mL, Sterile, DNase/RNase Tested, PCR Grade.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Quality Biological Inc TBE Buffer 10X, 1000 mL
TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. ¿ Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. ¿ Applications: TBE Buffer, 1X is used for DNA electrophoresis. ¿ TBE Buffer, 0.5X is used for electrophoresis onto DEAE cellulose membrane. ¿ TBE Buffer, 2X is used for polyacrylamide and agarose electrophoresis. ¿ TBE is also used for other nucleic acid electrophoresis procedures such as Northern and Southern blots. ¿ Storage Conditions: 15 to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 1L, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.